Gene and Enzyme relationship in the growth of Neurospora crassa:
Genes are a sequence of bases. Different genes have different base sequences. Most of the genes play a major role in the synthesis of their complimentary RNA. Some of this RNA is the structural part of organelles like Ribosomes, some function as tRNA molecules (these transport amino acids from the gene pool to the site of protein synthesis) and others as messengers (mRNA) for the protein synthesis.
A significant class of proteins is the enzymes molecules which carry out most of the chemical reactions in the cell. There are many types of enzymes in a cell; each one is capable to carry out a very specific metabolic function. The basic chemical subunits of the proteins are called as amino acids. There are 20 types or even more amino acids in number. In the structure of amino acids, these can be joined end to end by their common elements, an acidic end fusing with an alkaline end to form a peptide bond.
A linear string of amino acids connected together in this way is called as polypeptide. It is made up of many amino acids. Thus a protein consists of one or more polypeptide subunits bound together by weak chemical bonds.
One Gene-One Enzyme theory:
Beadle and Tatum working with Neurospora found that a heritable change in the structure of a gene can result in the absence of the activity of an enzyme. This observation leads to the finding of the relation between gene and enzyme. They also observed in one of their experiment that Neurospora (Bread mold) cells are able to survive and grow in an incomplete nutrient medium as it has biochemical pathways that can convert salts, sugar (sucrose), and biotin of the incomplete nutrient medium into major components needed by cells such as vitamins and amino acids, which are the essential components of a complete nutrient medium.
However the mutant forms of Neurospora will not grow in the minimal nutrient medium, they will require a vitamin (Thiamine) for their growth. In mutant forms, nutrient medium composition plays a major role in the growth of bread mold (Neurospora). An incomplete nutrient medium with no amino acids and enzymes leads to the improper growth of Neurospora but a complete nutrient medium with proper interaction of gene and enzyme leads to the growth of Neurospora crassa.
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