Experiments were carried out on male Wistar rats.
Experimental DM1 was induced by single streptozotocin administration (65 mg/kg, intraperitoneally) to
3-month old rats. Control animals received physiological saline (0.9% NaCl pH 4.5). DM1 development in
rats was associated with pronounced hyperglycemia
(22.1±1.0 mmol/liter), persistent glucosuria (28-56
mmol/liter) and polyuria. Neonatal DM2 was induced
by streptozotocin administration (80 mg/kg) to 5-day
old rat pups . In 2.5-3 months, DM2 sings appeared
detected using glucose tolerance test. Only rats with
glycemia 1.5-2-fold surpassing the normal after glucose load test (2 mg/kg) were included in the study.
Marked glucosuria (14-28 mmol/liter) was also noted
in rats with DM2.
To obtain heterogeneous cell membrane fraction
from rat brains, the cortex was homogenized in 1mM
buffer pH 7.5. Homogenate was centrifuged
at 8000g for 20 min (4o
C). Supernatant was diluted in
buffer and centrifuged at 80,000g for 30 min (4o
Precipitate was resuspended and used in subsequent
experiments. Protein concentration was measured by
the method of Bradford; γ-globulin was used for construction of the calibration curve.
Specifi c binding of 125I-insulin by rat brain cell
membranes was assayed by radioligand method with
plotting the curves of competitive displacement of
receptor-bind labeled insulin by unlabeled hormone.
The brain cell membrane fraction was incubated with
125I-insulin in the presence of 0.1-1000 ng/ml of unlabeled hormone. Non-specifi c binding was determined
by adding 10 μg/ml unlabeled insulin. Data of radioligand analysis was transformed into Scatchard coordinates to calculate KD and RО that characterize affi nity
and number of binding sites, respectively.
Expression of IRS2 protein gene was d
Are you busy and do not have time to handle your assignment? Are you scared that your paper will not make the grade? Do you have responsibilities that may hinder you from turning in your assignment on time? Are you tired and can barely handle your assignment? Are your grades inconsistent?
Whichever your reason may is, it is valid! You can get professional academic help from our service at affordable rates. We have a team of professional academic writers who can handle all your assignments.
Our essay writers are graduates with diplomas, bachelor's, masters, Ph.D., and doctorate degrees in various subjects. The minimum requirement to be an essay writer with our essay writing service is to have a college diploma. When assigning your order, we match the paper subject with the area of specialization of the writer.
- Plagiarism free papers
- Timely delivery
- Any deadline
- Skilled, Experienced Native English Writers
- Subject-relevant academic writer
- Adherence to paper instructions
- Ability to tackle bulk assignments
- Reasonable prices
- 24/7 Customer Support
- Get superb grades consistently
1. Place an order
You fill all the paper instructions in the order form. Make sure you include all the helpful materials so that our academic writers can deliver the perfect paper. It will also help to eliminate unnecessary revisions.
2. Pay for the order
Proceed to pay for the paper so that it can be assigned to one of our expert academic writers. The paper subject is matched with the writer’s area of specialization.
3. Track the progress
You communicate with the writer and know about the progress of the paper. The client can ask the writer for drafts of the paper. The client can upload extra material and include additional instructions from the lecturer. Receive a paper.
4. Download the paper
The paper is sent to your email and uploaded to your personal account. You also get a plagiarism report attached to your paper.
PLACE THIS ORDER OR A SIMILAR ORDER WITH SMART HOMEWORK HELP TODAY AND GET AN AMAZING DISCOUNT